Zakrzewska Lab


Jolanta Zakrzewska-Czerwińska


  • Since 2007: Head of Department of Molecular Microbiology, Faculty of Biotechnology, University of Wrocław,
  • 2002: Professor,
  • 1999 - 2014: Head of Department of Microbiology Institute of Immunology and Experimental Therapy, Polish Academy of Sciences,
  • Since 1996: Habilitation, Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland
  • 1991 - 1992: PostDoc, Alexander von Humboldt Fellowship, prof. Hilde Schrempf’s group, University of Osnabrueck, Germany,
  • 1989 Ph.D. supervisor: prof. Marian Mordarski, Biological Sciences, Institute of Immunology and Experimental Therapy, Wroclaw, Poland,
  • 1983: M.Sc. Bioengineering, Technical University of Wroclaw

room 3.20
phone: (+48)71-375-25-02
jolanta.zakrzewska(at)uwr.edu.pl

Chromosome replication and the regulation of this process

Replication is an essential process for every living cell. It is strictly regulated, mostly at the stage of replication initiation. Our team investigates mechanisms responsible for regulation of bacterial chromosome replication and analyzes real-time dynamics of this process at the single-cell level in various growth conditions.

Regulation of genes expression, including the genes involved in secondary metabolites (antibiotics) production

Actinomycetes are producers of many secondary metabolites of pharmaceutical importance, e.g. most clinically useful antibiotics, antifungals and anticancer drugs. However, most of these gene clusters for secondary metabolite synthesis are cryptic and silent under laboratory conditions. We study gene expression regulatory networks within gene clusters that are involved in the biosynthesis of secondary metabolites. Our task is to identify and subsequently awake these biosynthetic gene cluster. To reach the goal we apply the genome mining strategy that makes use of large number of genomic sequence data and molecular biology tools to activate biosynthetic gene clusters.


Team


Chromosome Replication

Damian Trojanowski

Project: Analysis of replisome dynamics during Mycobacterium smegmatis cell cycle


  • Since 2018: PostDoc, Department of Biotechnology, University of Wroclaw
  • 2017: PhD in Biochemistry, Department of Biotechnology, University of Wroclaw
  • 2009 – 2011: Engineering and technical assistant, Clinic of Infectious Diseases, Hepatology and Acquired Immune Deficiencies, Medical University of Wroclaw, Poland
  • 2008: M.Sc. Molecular Biology, University of Wroclaw, Poland
  • 2006: B.Sc. Biotechnology, University of Wroclaw, Poland

room 3.17
phone: (+48)71-375-23-93
damian.trojanowski(at)uwr.edu.pl

Joanna Hołówka

Project: The role of mIHF protein in Mycobacterium smegmatis chromosome organization and genes expression.


  • Since 2018: PostDoc, Department of Biotechnology, University of Wroclaw
  • 2018: PhD in Molecular Biology, Ludwik Hirszfeld Institute of Immunology and Experimental Therapy, Laboratory of Molecular Biology of Microorganisms
  • 2011-2012: MSc in Medicinal chemistry, Wroclaw University of Technology, Department of Chemisrty
  • 2007-2011: BSc in Biotechnology, Wroclaw University of Technology, Department of Chemisrty

room 3.17
phone: (+48)71-375-23-93
joanna.holowka(at)uwr.edu.pl

Marta Kołodziej

Project: The role of protein Lsr2 in maintaining chromosome structure and its participation in the regulation of cell cycle.


  • Since 2015: PhD. Student, Department of Molecular Microbiology, Faculty od Biotechnology, University of Wroclaw
  • 2015: M.Sc Biotechnology, Faculty od Biotechnology, University of Wroclaw
  • 2013: B.Sc. Biotechnology, Faculty od Biotechnology, University of Wroclaw

room 3.18
phone: (+48)71-375-26-40
marta.kolodziej(at)uwr.edu.pl

mgr inż. Hanna Kąkolewska

Project: The dynamics of the chromosome replication process in slow-growing Mycobacterium bovis.


  • Since 2018: PhD. Student, Department of Molecular Microbiology, Faculty od Biotechnology, University of Wroclaw
  • 2018: MSc in Farmaceutical Biotechnology, Wroclaw University of Technology, Faculty of Chemisrty
  • 2016: BSc in Biotechnology, West Pomeranian University of Technology, Faculty of Biotechnology and Animal Husbandry

room 3.18
phone: (+48)71-375-26-40
hanna.kakolewska2(at)uwr.edu.pl

Genes expression

Marcin Wolański

Projects:
  1. Regulation of chromosome replication in Streptomyces
  2. regulation of gene expression in biosynthetic gene clusters responsible for production of secondary metabolites in actinomycetes
  3. Bacterial response to environmental shock


  • 2011-2014, Since 2015: PostDoc Position - Department of Molecular Microbiology, Faculty of Biotechnology at University of Wrocław
  • 2011: Ph.D. from the Institute of Immunology and Experimental Therapy, Polish Academy of Sciences (PAS), Wrocław
  • 2005: M.Sc. from the Technical University of Wrocław

room 3.16
phone: (+48)71-375-63-16
marcin.wolanski(at)uwr.edu.pl

Tomasz Łebkowski

Project: Mechanisms of chromosome replication initiation in Streptomyces coelicolor


  • Since 2013: PhD Candidate - University of Wroclaw Candidate, Molecular biology
  • 2013: M.Sc Biotechnology - Faculty od Biotechnology, University of Wroclaw, Specialization: Biotechnology of peptides and proteins,
  • 2011: B.Sc. Biotechnology - Faculty od Biotechnology, University of Wroclaw

room 3.17
phone: (+48)71-375-23-93
tomasz.lebkowski(at)uwr.edu.pl

Bdellovibrio bacteriovorus

Łukasz Makowski

Project: Characteristic of the chromosome replication process in predatory bacterium Bdellovibrio bacteriovorus


  • Since 2013:2013: PhD Student, Institute of Immunology and Experimental Therapy,  Polish Academy of Sciences
  • 2013: M. Sc. Molecular Microbiology, University of Wroclaw
  • 2011: B. Sc. Biotechnology, Faculty of Biotechnology, University of Wroclaw

room 3.18
phone: (+48)71-375-26-40
lukasz.m.makowski(at)gmail.com