Chromosome replication and the regulation of this process
Replication is an essential process for every living cell. It is strictly regulated, mostly at the stage of replication initiation. Our team investigates mechanisms responsible for regulation of bacterial chromosome replication and analyzes real-time dynamics of this process at the single-cell level in various growth conditions.
Regulation of genes expression, including the genes involved in secondary metabolites (antibiotics) production
Actinomycetes are producers of many secondary metabolites of pharmaceutical importance, e.g. most clinically useful antibiotics, antifungals and anticancer drugs. However, most of these gene clusters for secondary metabolite synthesis are cryptic and silent under laboratory conditions. We study gene expression regulatory networks within gene clusters that are involved in the biosynthesis of secondary metabolites. Our task is to identify and subsequently awake these biosynthetic gene cluster. To reach the goal we apply the genome mining strategy that makes use of large number of genomic sequence data and molecular biology tools to activate biosynthetic gene clusters.