Chromosome segregation and its coordination with cell division and other cell cycle processes
We focus on the function of the chromosome segregation proteins ParA and ParB in Streptomyces and Mycobacterium . We found that ParA interacts with polar proteins, Scy in Streptomyces and DivIVA in Mycobacterium. Studying the ParA and ParB interactions we explore the coordination of chromosome segregation with the other cell processes - cell elongation and cell division.
The function of topoisomerases and NAPs in chromosome organisation during bacterial cell cycle
Chromosome topology is dependent on the function of topoisomerases and NAP (nucleoid associated proteins). We found that in Streptomyces high topoisomerase I (TopA) processivity is essential during chromosome segregation. Now, we further explore the TopA mediated chromosome organisation during cell cycle and coordination of TopA activity with the function of other DNA binding proteins.