Arkadiusz Kozubek                 

Professor

Mailing address: Institute of Biochemistry and Molecular Biology, University of Wroclaw,

 Department of Lipids and Liposomes, Przybyszewskiego 63/77,
 51-148 Wroclaw, Poland
Office Number: 314
Email address: kozubek@ibmb.uni.wroc.pl
Fax address: 48 (+71) 3 756 234
Phone Number: 48 (+71) 3 756 204
 

Academic degrees

• MSc: University of Wroclaw, biochemistry >
• PhD: University of Wroclaw, biochemistry. Thesis: "Lysine-rich histones from various animal tissues"

with Prof. dr hab. Wanda Mejbaum-Katzenellenbogen, 1973
• Habilitation (Dr hab.): University of Wroclaw, biochemistry/biophysics. Monograph: "The effect of some non-isoprenoic phenolic lipids with biological membranes", Wroclaw University Press 1986
• Full Professor 1998
• Postdoctoral Experience: with Rudolf A. Demel and Wouter Geurts van Kessel (Rijksuniversiteit Utrecht, Nederland, 1979-80) Studies concerned alkylresorcinol-lipid bilayer interactions; with Mahendra K. Jain (Delaware State University, 1985-86) Studies concerned the effect of amphiphiles on interfacial kinetics of phospholipase A₂.

FEBS Fellow (Rijksuniversiteit Utrecht, Nederland, 1982) Studies on plant phenolic lipids isolation and characterization
IMG TEMPUS (Rijksuniversiteit Utrecht, Nederland, 1991) Collaboration with Ron van't Hof and Ben de Kruijf in studies on the role of plant lipids on protein import to chloroplast envelope.
EC COST Fellow (Brunel University, London, 1993) Collaboration with John H.P. Tyman in studies on chemical synthesis and analysis of resorcinolic lipids.

Areas of Involvement

Teaching
 
• Biochemistry
• Lipids and Biological Membranes
• Liposome Technology
• Biochemistry for Biology Teachers
• BSc and MSc Seminars

Textbooks (in Polish):
Laboratory Course of Biochemistry, (Editor: W.Mejbaum-Katzenellenbogen) Wroclaw University Press, 1981, Second Edition 1985, Third Edition 1989, Revised Editions 1992, 1994
Molecular Organization of the Cell. Laboratory Textbook (Editor: J. Szopa) Wroclaw University Press 1992, Second Edition 1996
Molecular Organization of the Cell, II. Lipids, Liposomes and Biological Membranes. Laboratory Textbook (Editor: A. Kozubek) Wroclaw University Press 1993, Second Edition 1995, Third Edition 1997


Administration
 

• Vice-Director of the Institute of Biochemistry (1988-1989)
• Head of the Laboratory Biological Membranes (1985-1997)
• Head of the Department of Lipids and Liposomes (1998-)
• Member of the University Senate Commission for Research and Teaching Staff (1984-1986)
• Member of the University Senate Financial Commision (1993-1996)
• Institute coordinator of ERASMUS/SOCRATES programme (1997-
• Vice-Director of the Institute of Biochemistry and Molecular Biology (1999-2002)

 
Professional Activities
 
• Member of European Association of Pharma Biotechnology
• Member of Polish Biochemical Society
• Member of Polish Biophysical Society
• Member of Molecular Biology Commision, Wroclaw Branch of Polish Academy of Sciences
• Co-Editor of Cellular and Molecular Biology Letters
• Member of the W. Mejbaum-Katzenellenbogen's Molecular Biology Seminars Organizing Committee
• Reviewer for many journals and KBN grant reviewer

Current Research interests

In looking at my research program since 1973, I realize that I am involved mostly in many various aspects of  resorcinolic lipids in cereals, from isolation, characterization, analysis and determination to their biological activities and recently biosynthesis and physiological role. Although at present my research topics are focused in establishing some general scheme of  mutual relationship between lipid and phenolic lipid metabolism in plant and bacterial cell, the studies on the further characterization of these compounds and their biological activities as well as the role of lipids, in general,  in modulation of the cellular functioning are continued.
Other important topic deals with research on liposomes as drug carriers. It includes research on establishing new formulations of drug-delivering liposomes with the use of natural amphiphilic modifiers.
Student projects involves whatever is required to reach a solution to above problems, including studies of cell and bacterial cultures, biochemistry, natural products chemistry, molecular biology and genetic regulation.
 

Current Collaborations

Prof. Gerrit L. Scherphof; University of Groningen, Groningen, The Netherlands,
Prof. Dick Hoekstra, University of Groningen, Groningen, The Netherlands,
Prof. Gert Storm, University of Utrecht, Utrecht, The Netherlands,
Prof. Afaf Kamal-Eldin, Swedish University of Agricultural ciences, Uppsala, Sweden,
Prof. Martin Hof, Czech Academy of Sciences, Prague, Czech Republic,
Prof. Paavo Kinnunen, University of Helsinki, Helsinki, Finland,
Dr. John H.P. Tyman, University of West London, Uxbridge, United Kingdom,
Prof. Marek Langner, Technical University Wroclaw, Wroclaw, Poland,
Prof. Maciej Ugorski, Agricultural University, Wroclaw, Poland

Recent publications (for full text PDF files see department page - http://www.ibmb.uni.wroc.pl/lip4.htm)

• Modification of the lipid component modulates nuclear matrix nucleolytic activity.

Joanna Lubocka, Jan Szopa, Arkadiusz Kozubek
Acta Biochim. Polon. 42 (1995) 211-216
• Modulation of some nuclear matrix enzymatic activities by free fatty acids.

Joanna Lubocka, Jan Szopa, Arkadiusz Kozubek
Acta Biochim. Polon. 42 (1995) 217-220
• Interaction of alkylresorcinols with proteins.

Arkadiusz Kozubek
Acta Biochim. Polon. 42 (1995) 241-246
• Determination of octanol/water partition coefficients for long-chain homologs of orcinol from cereal grains.

Arkadiusz Kozubek
Acta Biochim. Polon. 42 (1995) 247-252
• Cereal grain resorcinolic lipids inhibit H₂O₂-induced peroxidation of biological membranes.

Arkadiusz Kozubek, Barbara Nienartowicz
Acta Biochim. Polon. 42 (1995) 309-316
• Isolation of resorcinolic lipids from cereal grain extracts by use of a non-aqueous bi-phasic solvent system.

Arkadiusz Kozubek
Nat. Prod. Lett. 7 (1995) 123-128
• Fusiogenic activity of natural amphiphiles, 5-n-alkylresorcinols in a yeast protoplast system.

Arkadiusz Kozubek, Jacek Skala
Z. Naturforsch. 50c (1995) 656-659
• Cereal grain resorcinolic lipids: mono and dienoic homologues are present in rye grains.

Arkadiusz Kozubek, John H.P. Tyman
Chem. Phys. Lipids 78 (1995) 29-35
• Modulation of hemolytic properties of resorcinolic lipids by divalent cations.

Maria Stasiuk, Arkadiusz Kozubek
Cell. Mol. Biol. Lett. 1 (1996) 189-198
• Alkylresorcinols are abundant lipid components in different strains of Azotobacter chroococcum and Pseudomonas spp.

Arkadiusz Kozubek, Stanislaw Pietr, Anna Czerwonka
J. Bacteriol. 178 (1996) 4027-4030
• Antimutagenic activity of alkylresorcinols from cereal grains.

Kazimierz Gasiorowski, Katarzyna Szyba, Barbara Brokos, Arkadiusz Kozubek
Cancer Lett. 106 (1996) 109-115
• Modulation of 5-n-alkylresorcinol hemolytic properties by divalent cations. Dependence of the effect of cations on alkylresorcinol structure.

Maria Stasiuk, Arkadiusz Kozubek
Cell. Mol. Biol. Lett. 2 (1997) 77-87
• Inhibition of soybean lipoxygenases by resorcinolic lipids from cereal bran.

Luiza Deszcz, Arkadiusz Kozubek
Cell. Mol. Biol. Lett. 2 (1997) 213-222
• Long-chain orcinol homologs from cereal bran are effective inhibitors of glycerophosphate dehydrogenase.

Joanna Rejman, Arkadiusz Kozubek
Cell. Mol. Biol. Lett. 2 (1997) 411-419
• Modulation of plant nuclear matrix nucleolytic activity and specificity may result from interaction of nuclease-protein 14-3-3 complex with inner nuclear membrane lipids.

Joanna Lubocka, Arkadiusz Kozubek
Cell. Mol. Biol. Lett. 3 (1998) 57-73
• Quantum mechanical and experimental oxidation studies of pentadecylresorcinol, olivetol, orcinol and resorcinol.

Jerzy Hladyszowski, Ligia Zubik, Arkadiusz Kozubek
Free Radical Res. 28 (1998) 359-368
• Resorcinolic lipids, the natural amphiphiles and their biological activity.

Arkadiusz Kozubek, John H.P. Tyman
Chem. Rev. 99 (1999) 1-26
• Alkylresorcinol homologs in seeds of Pisum sativum varieties.

Robert Zarnowski, Arkadiusz Kozubek
Z. Naturforsch. 54c (1999) 44-48
• Dual effect of alkylresorcinols, natural amphiphilic compounds, upon liposomal permeability.

Jerzy Gubernator, Maria Stasiuk, Arkadiusz Kozubek
Biochim. Biophys. Acta 1418 (1999) 253-260
• Effect of rye 5-n-alkylresorcinols on in vitro growth of phytopathogenic Fusarium and Rhizoctonia fungi.

Robert Zarnowski, Arkadiusz Kozubek, Stanislaw Pietr
Bull. Pol. Acad. Sci. 47 (1999) 231-235

• Higher cardol homologues (5-alkylresorcinols) in rye seedlings.

 Luiza Deszcz, Arkadiusz Kozubek
 Biochim. Biophys. Acta 1483 (2000) 241-250
• Alkylresorcinols in Fruit Pulp and Leaves of Ginkgo biloba L.

 Robert Żarnowski, Ewa D. Żarnowska, Arkadiusz Kozubek
Z. Naturforsch. 55c (2000) 881-885
• Alkylresorcinols in the Family Fabaceae.

 Ewa D. Żarnowska, Robert Żarnowski, Arkadiusz Kozubek
 Acta Soc. Bot Polon. 70 (2001) 25-29
• Formation of Liposomes by Resorcinolic Lipids, Single-Chain phenolic Amphiphiles from Anacardium occidentale L.

 wa Przeworska, Jerzy Gubernator, Arkadiusz Kozubek
 Biochim. Biophys. Acta 1513  (2001) 75-81